The high esterification activities rate of rice bran lipase at 37 C were found in free fatty acid and butanol as substrate in 1.338 mole ratio at reaction time 30 minutes in water saturated isooctane as solvent. The composition of fatty acid butyl ester were detected by gas chromatographic technique which indicate that the specification of lipase to saturated and unsaturated of short chain to long chain fatty acids.
Keywords : Esterification activity, Lipase, organic solvent