Sampling
Altogether 432 samples were used for this
study. Between 2005 and 2007, 330 individuals
were captured on localities Hylváty, Zabitý, Horní
Morava, and Zhoř, and it we took the last two
phalanges from the longest fi nger of the le front
limb (group A). Between 2008 and 2009, 102 newts
were captured on localities Zabitý, Horní Morava,
and Zhoř. We severed the whole longest fi nger
from the le front limb of each of these individuals
(group B). Sampling was done with nail scissors
and without anesthetization. A er each individual
sampling, the scissors were disinfected in 70%
alcohol. The removed digits were preserved in 70%
alcohol and stored in labelled tubes (Figs. 1C, 1D).