To the best of our knowledge, soymilk or soybean seeds were
commonly the sources used in previous reports where
isoflavones were studied (Marazza et al., 2012; Singh et al., 2010),
but little has been understood for the isoflavones in the soy
whey. In the present investigation, four major isoflavones,
namely, daidzin, genistin, daidzein and genistein were identified
in the soy whey extracts before and after fermentation,
as monitored by HPLC analysis (Table 1 and Fig. 2). As shown
in Table 1 and Fig. 2, aglycone concentration (genistein and
daidzein) in FSW significantly increased during fermentation,
daidzein and genistein were significantly increased
(p < 0.05) to 5.15 ± 0.01 and 4.68 ± 0.02 mg/g, respectively, which
were about 4.5- and 4.1-fold higher, respectively, compared to
the USW. This high level obtained for daidzein and genistein
was expected, due to the high initial daidzin and genistin concentration
in the studied soy whey, which were 14.30 ± 0.30 and
27.61 ± 0.44 mg/g, respectively, in the soy whey. Table 1 also indicated
that phenolic contents obtained from HPLC analysis
were much lower than that obtained from TPC, this was possibly
due to the fact that some of the minor phenolics
compounds were not identified in the HPLC analysis.
To the best of our knowledge, soymilk or soybean seeds werecommonly the sources used in previous reports whereisoflavones were studied (Marazza et al., 2012; Singh et al., 2010),but little has been understood for the isoflavones in the soywhey. In the present investigation, four major isoflavones,namely, daidzin, genistin, daidzein and genistein were identifiedin the soy whey extracts before and after fermentation,as monitored by HPLC analysis (Table 1 and Fig. 2). As shownin Table 1 and Fig. 2, aglycone concentration (genistein anddaidzein) in FSW significantly increased during fermentation,daidzein and genistein were significantly increased(p < 0.05) to 5.15 ± 0.01 and 4.68 ± 0.02 mg/g, respectively, whichwere about 4.5- and 4.1-fold higher, respectively, compared tothe USW. This high level obtained for daidzein and genisteinwas expected, due to the high initial daidzin and genistin concentrationin the studied soy whey, which were 14.30 ± 0.30 and27.61 ± 0.44 mg/g, respectively, in the soy whey. Table 1 also indicatedthat phenolic contents obtained from HPLC analysiswere much lower than that obtained from TPC, this was possiblydue to the fact that some of the minor phenolicscompounds were not identified in the HPLC analysis.
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