Figure 2. SdFFF fractogram of 70-nm SiO2 particles recovered from acid-digested rat liver.
To demonstrate the capability to distinguish nano-sized (diameter < 100 nm)
and submicron particles alone and in lung tissue, mixtures of two different sizes
of SiO2 particles were added either to aqueous surfactant (reference sample) or
added to homogenized lung tissue which was processed by the tissue digestion
procedure. Figure 3A shows light scattering versus time from SdFFF analysis of
the reference sample normalized to the largest peak. This sample contains the
70- and 250-nm manufactured SiO2 particles at a 2:1 mass ratio in aqueous surfactant.