Introduction
Transition step between dehydration and infiltration with embedding medium is often referred to as clearing or de-alcoholization (Avwioro, 2002). It is a process that requires the use of solvents known as clearants or clearing agents that are miscible with both absolute alcohol and the embedding medium. Alcohol is mostly used as a dehydrating agent before de-alcoholization in a clearing agent, other dehydrating agents such as acetone and dioxane (diethylene dioxide) can also be used