which showed Meyer 311
(GenBank ID JQ638805) to be a 100% match to S. viarum accession
AY561275 used in the Solanum phylogenetic study by Levin et al.
(2006).14 Two fruit from each plant of Meyer accession 311 were
harvested at the mature unripe stage, i.e., full size but mottled green
and white in color rather than yellow. The four fruit were pooled as a
single sample, frozen in liquid N2, and stored at −80 °C. The frozen
tissue was lyophilized and powdered with a mortar and pestle prior to
extraction