Osmotic agents (osmolytes)
osmolytes are low-molecular-weight substances
they stabilize and enhance protein solubility
examples: sucrose, trehalose, sorbitol, glycine
mechanisms of action: preferential exclusion
Stabilizers
Wide variety of stabilizer is in use: amino acids, sugars, polyols
amino acids, particularly Gly, reduces protein adsorption, inhibits aggregation, stabilizes protein conformation against heat denaturation.
other use of sugar is being bulking agent for freeze-drying process
Osmotic agents (osmolytes) osmolytes are low-molecular-weight substances they stabilize and enhance protein solubility examples: sucrose, trehalose, sorbitol, glycine mechanisms of action: preferential exclusion Stabilizers Wide variety of stabilizer is in use: amino acids, sugars, polyols amino acids, particularly Gly, reduces protein adsorption, inhibits aggregation, stabilizes protein conformation against heat denaturation. other use of sugar is being bulking agent for freeze-drying process
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