it is likely that
the detergent mimics the native environment by creating micelles
that stabilize the target protein. In fact, little precipitate formation
was observed in the sample after the addition of DDM. Solubilized
recombinant protein gave a single heme-stained band on SDS–
PAGE gel between the 35 kDa and 48 kDa markers (Fig. 1B_lane
a). Coomassie blue confirmed the purity of sample by revealing
the absence of any other protein (Fig. 1B_lane b).