Acetylcholine is a neurotransmitter active in central nervous systems and skeletal-muscle junction. Acetylcholine esterase (AChE) is the hydrolase that degrades acetylcholine molecules into choline and acetic acid, thus terminating impulse transmission at cholinergic synapsis. Therefore, AChE controls generation of nerve impulses in the postsynaptic neurons. Toxicity of OP and CM depends on inhibition of AChE; thus the enzyme is a common bioevaluator for the detection of organophosphates and carbamates [7]. Extention of inhibition of this enzyme has been frequently used to measure quantitatively the presence of such pesticides. AChE inhibition has been proven to be useful in monitoring organophosphate and carbamate changes in samples with various platforms of sensors including surface plasmon resonance (SPR) [8], electrochemical [9, 10], colorimetric [11], various nanomaterials-based methods [12–15]. However, AChE is the most widely used biorecognition element in biosensor development for pesticide detection [7]. Paper matrix has been the improved material for some AChE based detection devices such as sol-gel entrapment of gold nanoparticles for paper-dipstick sensor device [16], a lateral flow application [17]. Moreover, microfluidic paper devices could be developed by patterning hydrophilic channels and hydrophobic barriers [18]. Thus, paper could be a lowcost ubiquitous material for developing alternative multiplex sensors for onsite pesticide determination for medical diagnostics, environmental monitoring, or food quality analysis.