Direct plating on VVA and CPC+ followed by colony hybridization
was utilized to enumerate V. vulnificus from56 oyster samples collected
from May to December, 2007. Estimates obtained using either media
demonstrated similar regional and seasonal trends of total V. vulnificus
levels (Fig. 1). Highest mean levels each season were found in oysters
harvested from the Gulf Coast: 3.05 and 3.15 log CFU/g from May
to Jun, 3.64 and 3.61 log CFU/g from Jul to Sep, and 2.09 and
1.71 log CFU/g fromOct to Dec using VVA and CPC+media, respectively.
Mean levels in Atlantic Coast oysters were 2.73 and 2.23 log CFU/g
from May to Jun, 1.95 and 1.87 log CFU/g from Jul to Sep, and 1.75 and
1.40 log CFU/g fromOct to Dec using VVA and CPC+media, respectively.
Pacific Coast oysters hadmean levels of 1.96 and 0.80 log CFU/g from
May to Jun, 1.37 and 1.42 log CFU/g from Jul to Sep, and 1.60 and
1.60 log CFU/g from Oct to Dec samples from VVA and CPC+ media,
respectively.
Direct plating on VVA and CPC+ followed by colony hybridizationwas utilized to enumerate V. vulnificus from56 oyster samples collectedfrom May to December, 2007. Estimates obtained using either mediademonstrated similar regional and seasonal trends of total V. vulnificuslevels (Fig. 1). Highest mean levels each season were found in oystersharvested from the Gulf Coast: 3.05 and 3.15 log CFU/g from Mayto Jun, 3.64 and 3.61 log CFU/g from Jul to Sep, and 2.09 and1.71 log CFU/g fromOct to Dec using VVA and CPC+media, respectively.Mean levels in Atlantic Coast oysters were 2.73 and 2.23 log CFU/gfrom May to Jun, 1.95 and 1.87 log CFU/g from Jul to Sep, and 1.75 and1.40 log CFU/g fromOct to Dec using VVA and CPC+media, respectively.Pacific Coast oysters hadmean levels of 1.96 and 0.80 log CFU/g fromMay to Jun, 1.37 and 1.42 log CFU/g from Jul to Sep, and 1.60 and1.60 log CFU/g from Oct to Dec samples from VVA and CPC+ media,respectively.
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