The fixative of BF was recommended to study reproductive toxicity by the Organization for Economic Cooperation and Development (OECD) and the International Conference on Harmonization (ICH), for its roles in cellular preservation and the higher resolution of cellular details [18,19]. However, picric acid in BF fixative caused health hazards, disposal problems, bright yellow staining work surfaces and requiring lots of alcohol rinses to remove picric acid for later testicular examination [20,21].