Effect of beta-mercaptoethanol (bME) on viability (A) of
frozen-thawed boar sperm and acrosomal functional status (B) of the
live cells as determined by Hoechst 33,258 fluorescence and chlortetracycline
(CTC) fluorescence patterns (F, intact cells; Cap, capacitated
cells; AR, acrosome-reacted cells), recpectively. Frozenthawed
sperm were cultured in the absence or presence of 25 and 50
M bME in the thawing solution together with caffeine for 90 min