With 16 pairs of primer combinations, 54 mutation sites were observed in strain 971-5, 86 in strain 972-4, and 5 in strain R955 compared to their controls, and their mutation rates were 4.3%, 6.9% and 0.4%, respectively [Formula (2)]. Of the 54 mutation sites found in strain 971-5, and 86
found in strain 972-4, 75.9% and 84.9% of the mutations occurred at polymorphic sites,respectively [Formula (3)], and the remaining mutations occurred in the conserved regions (Fig. 1). All 5 mutation sites found in R955 occurred at polymorphic sites with the consistency rate
being 100% [Formula (3)].
With 16 pairs of primer combinations, 54 mutation sites were observed in strain 971-5, 86 in strain 972-4, and 5 in strain R955 compared to their controls, and their mutation rates were 4.3%, 6.9% and 0.4%, respectively [Formula (2)]. Of the 54 mutation sites found in strain 971-5, and 86found in strain 972-4, 75.9% and 84.9% of the mutations occurred at polymorphic sites,respectively [Formula (3)], and the remaining mutations occurred in the conserved regions (Fig. 1). All 5 mutation sites found in R955 occurred at polymorphic sites with the consistency ratebeing 100% [Formula (3)].
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