luminescence decays
in samples containing a suspension of a thylakoid D2O-phosphate
buffer, are shown at 1270 nm, 1355 nm and with NaN3, after excitation
with 420 nm microjoule pulses. All three luminescence decay
curves exhibit significant intensity right after the laser pulse; this
could be attributed to the residual fluorescence of the photosynthetic
system and, perhaps, to a scattering of the laser light.
However, at longer times, particularly between 10 and 50microseconds,
the two decay curves recorded at 1355 nm and in the presence
of NaN3 significantly differ from the one recorded at 1270 nm. The
higher luminescence intensity in this time domain, observed at
1270 nm, is due to singlet oxygen phosphorescence, which shows
an apparent lifetime of ≈55 s. The measurement of singlet oxygen
phosphorescence was carried out in a D2O-phosphate buffer,
in which the singlet oxygen lifetime was reported to be 50–60 s
luminescence decaysin samples containing a suspension of a thylakoid D2O-phosphatebuffer, are shown at 1270 nm, 1355 nm and with NaN3, after excitationwith 420 nm microjoule pulses. All three luminescence decaycurves exhibit significant intensity right after the laser pulse; thiscould be attributed to the residual fluorescence of the photosyntheticsystem and, perhaps, to a scattering of the laser light.However, at longer times, particularly between 10 and 50microseconds,the two decay curves recorded at 1355 nm and in the presenceof NaN3 significantly differ from the one recorded at 1270 nm. Thehigher luminescence intensity in this time domain, observed at1270 nm, is due to singlet oxygen phosphorescence, which showsan apparent lifetime of ≈55 s. The measurement of singlet oxygenphosphorescence was carried out in a D2O-phosphate buffer,in which the singlet oxygen lifetime was reported to be 50–60 s
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