The
cassette containing the double 35S promoter from Cauliflower
mosaic virus (CaMV), enhancer sequence from Alfalfa
mosaic virus, gus::nptII fusion, and nos terminator from the
plasmid pBI426 [15] was excised with HindIII and EcoRI
blunt ended and ligated into the filled-in XbaI restriction site
of a pBS II KS+ (Stratagene Inc., La Jolla, CA, USA).