Seven healthy Murrah buffalo bulls (4-6 years age) with
good body condition (score 5-6) maintained under uniform
feeding, housing, lighting and management conditions at
Germplasm Centre, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India, is located at an altitude of
564 feet above the sea level and at latitude of 28曘 north
and a longitude of 79曘 east. The climate touches both the
extremes of cold and hot weather experienced in the country
and the relative humidity ranges between 15% and 85%.