2.6. Total phenols determination
Total phenols were measured according to Velioglu et al.(1998) using FolioCiocalteu reagent. Two hundred milligrams of fruit tissue (including skin and flesh) were extracted with 2 ml of 50% methanol for 2 h at ambient temperature. The mixture was centrifuged for 10 min and the supertanker was decanted into 4 ml vials. A 200 l of the extract was mixed with 1.5 mlFolinCiocalteu reagent (previously diluted 10-fold with distilled water) and allowed to stand for 5 min before the addition of 1.5 ml of 20% sodium carbonate. After 90 min, absorbance was measured at 750 nm using a UV-Vis Spectrophotometer. The blank contains only water and the reagents. Total phenols were quantified from a calibration curve obtained by measuring the absorbance of known concentrations of gallic acid.