Actinomycete mat (20 gm) was obtained from actinomycete broth and used for the synthesis of silver nanoparticles. The harvested cell filtrate was suspended in sterile double distilled water for 1 day. After one day incubation, the cell filtrate (biomass) was obtained by passing it through Whatman filter paper. 20 gm of biomass (fresh weight) was grind well using mortar and pestle and mixed with 200 ml of Millipore water in a 500 ml Erlenmeyer flask and agitated in the same condition for 72 hour at 37oC. The same was repeated for the cell free filtrate (filtered water) along with experimental flask (20 ml filtered water in 200 ml of Millipore water). Biomass and water samples were randomly named as B and W. The biomass strains were named as BP1, BP8 and BNP1. The filtered water samples were named as WP1, WP8 and WNP1.For the synthesis of silver nanoparticles, 50 ml of 1 mM AgNO3 solution was mixed with 50 ml of cell filtrate in a 250 ml Erlenmeyer flask and agitated at 37oC in darker condition. The same was done for the cell free filtrate (50 ml of 1 mM AgNO3 solution with 50 ml filtered water). Simultaneously, control without silver ions was also run along with the experimental flasks. Colour change was noted at specific intervals (12 hours and 72 hours). The nanoparticles were characterized by UV-visible spectroscopy and Scanning Electron Microscope (SEM) analysis.