Fig. 2 Influence of epicatechin on the appressorial melanization
of Colletotrichum kahawae Z1 and on the
symptom expression on ripe and green berries. (I) Conidia
germinated in distilled H2O (control; a, indicating appressorium;
c, indicating conidium); (II) inoculated conidia
containing 1.2 mg epicatechin/ml; (III and IV) symptoms
expression on ripe and green berries, respectively, inoculated
conidia containing 0 (A and D), 1.2 (B and E) and
2.3 mg (C and F) epicatechin/ml. Note that conidia treated
with epicatechin produced unmelanized appressoria,
resulting in delayed or no infection at all on ripe and
green berries. Symptoms were evaluated at d 7 (ripe
berries) and d 10 (green berries) after inoculation,
respectively. Inoculated zones were cycled with black
pen on ripe berries. Similar results were obtained on the
berries inoculated with conidia containing the same
concentration of (+)-catechin