These peroxidase substrates are
commonly used in enzyme-linked immunosorbent assays (ELISA),
The reaction mixture with a final volume of 1.0 mL contained a concentration of 1 mM H2O2, 10 lL of HRP isoenzyme preparation and
varying concentrations of ABTS (0.05–10 mM) or TMB (0.005–
0.5 mM) in 50 mM potassium phosphate buffer, pH 6.5.