Positive control from the kit and
reaction control containing all the reaction mixture except
tem plate was included in each run. The cycling conditions included
an initial pre-incubation step of 95 C for 10 min followed by 45
cycles of denaturation at 95 C for 20 s, annealing at 60 C for 25 s
and extension at 72 C for 15 s. Data analysis was performed using
Light Cycler data analysis software ver. 3.5.5 using fit point
algorithm.