Functional xylooligosaccharides (XOS) from wheat bran were carried out by screening and cellulase
assisted enzymatic hydrolysis. First, wheat bran was crushed and screened, getting the optimal experimental
particle size of raw materials (>125 m), and 72.43% of starch. Wheat bran was then repeated
colloid milled and hydrolysed with cellulase, releasing about 45% of xylan (higher than the untreated
wheat bran (about 29%)). Finally, membrane treatment (20 kDa and 1 kDa cutoff) was chosen to eliminate
impurities and enriched XOS. In the designed process, 51 mg purified XOS was obtained from 1 g
dry wheat bran powder.