Besides the official methodology, several methods have been described for detection and determination of
proteolytic activities [9] [10]. Among them, the simplest and most accessible method uses colorimetric reactions,
which are detected and quantified by spectrophotometry [6] [11] [12]. The main advantage of spectroscopy is
being quantitative, at a low operational cost and easy to use [13].
Megazyme Company has several analytical kits for this purpose including Protazyme AK tablets [14]. Megazyme
suggests a quantification method for protease using its tablets. In this work, some adaptation of this method
using different reagents that are more available locally as well as less costly are shown.