2.5. Molar ratio optimization of lipase catalyzed reaction
Different amounts of methanol (1:3, 137 lL; 1:4, 162 lL; 1:5,227 lL;1:6, 274 lL; and 1:10, 457 lL) were added to the mixture consisting of waste lipid (1 g) and lipase (0.1 g, 10 wt.%). The
temperature was kept constant at 40 _C. After 6 h of reaction,the samples were separated from the enzyme by filtration. The resulting samples were then dried and analyzed by 1H NMR afterwards.