Fig. 3 reveals the fungal growth incidence on bread samples packed with pure chitosan and chitosan-based composite films.The sample packed in low-density polyethylene (LDPE) served as the control. Visible fungal growth appeared on the surface of the control sample after 3 days of storage at 24 C. Delayed development of fungal was observed in all samples packed with pure chitosan and chitosan-based composite films. Fungal growth
was detected on the pure chitosan film (CG0) packed sample on day 5. The antifungal activity of chitosan-based composite films notably increased with an increasing content of GFSE, with fungal growth only observed on day 8 for the sample packed with 0.5% v/v GFSE chitosan-based film (CG1), day 9 for the sample packed with 1.0% v/v GFSE chitosan-based film (CG2) and subsequently on day,10 for the sample packed with 1.5% v/v GFSE chitosan-based film(CG3). Results from the preliminary antifungal activity evaluation
indicated that the incorporation of GFSE into pure chitosan film improved the antifungal properties of the film and significantly retarded the proliferation of fungal on bread samples.