a melting curve was generated for the PCR product; the samples were cooled to 60 ◦C (20 ◦C per second), followed by heating in steps of 0.2 ◦C per second up to 95 ◦C
a melting curve was generated for the PCR product; the sampleswere cooled to 60 ◦C (20 ◦C per second), followed by heating insteps of 0.2 ◦C per second up to 95 ◦C