The recombinant protein waspurified with Glutathione Sepharose 4B (GE Healthcare). The EMSAwas performed using the EMSA kit (Thermo) according to themanufacturer's instructions. The probes containing A[C/G]Tderived from MaACS1 and MaACO1 promoters were labeled withbiotin using DNA 3' End Biotinylation Kit (Thermo)