Material and methods
2.1. Materials
Reed used in this study was friendly provided by Yingkou
Papermaking Mill, Liaoning Province, China. The one-year old
reed was harvested in fall and air-dried at room temperature
in our laboratory. The reed was mechanically milled with a lab
mill (Ultra Centrifugal Mill ZM 200, Retsch GmbH, Germany)
and sieved through standard mesh sieves (200e500 mm) to
obtain the powder of 200e500 mm particle sizes. The commercial
Trichoderma reesei cellulase (Celluclast 1.5 L) with
FPase activity of 187 IU/mL and b-glucosidase activity of 32 IU/
mL was provided by Novozymes China.