The appearance of CPE was more rapid in SRTF
than in GFF cell cultures, and the sensitivity of SRTF
cells to CyHV-2 was higher than GFF cells (data not
shown). However, the cost of maintaining SRTF cells
is higher than GFF cells, since SRTF requires the
flask to be coated with collagen, which is expensive,
and the sub-culture split ratio is only 1:2. Therefore,
GFF cells were used for the preparation of inocula for
experimental infections in this study. In addition,
propagation of the virus at 15, 20 and 25°C was
investigated in advance, and it was observed that the
completion was most rapid at 25°C. Consequently,
CyHV-2 was propagated using GFF cells sustainably
beyond 12 passages by the method described in
‘Materials and methods’.