Preparation of dry starter cultures and sato fermentation
The isolated yeasts, Y01, Y02, Y03 were separately cultured in YM broth
for 5 days. After centrifugation at 3,000 rpm, yeast cells were washed
with sterilized distilled water. The cell suspension was prepared at about
1.0 x 108 cells/ml. The spore suspension of two isolated fungi, Aspergillus
sp. and Rhizopus sp. were prepared similarly at about 2.0 x 107 spores/ml.
After mixing the culture at different mixing combinations (Table 1), ten
grams of sterilized dry rice flour (12% moisture content) was added to the
mixture to make dry starter culture