High performance liquid chromatography data analysis
The mobile phase comprised acetonitrile/water – 2% acetic acid (70:30 v/v). This is the result of several trials that were
performed to find the conditions that provided the extraction.
The curcumin standard revealed a clear peak at 3.2 min, and the area under peak was considered as 100% curcumin,
equivalent to 100 μg/mL. The sample of aqueous extract of C. longa showed few peaks. The largest area under a curve
showed curcumin to be the major component of turmeric,with a concentration of 42 μg/mL. However, a peak tailing
was identified in the C. longa chromatogram, which could be due to an overlap of 2 peaks (Fig. 1c).