2.5. Statistical analysis of data
Data were analysed through two-way ANOVA with an a: 0.05 and Tukey was the post-hoc test applied. Results are reported based on their mean and standard deviation (Sokal & Rohlf, 2000). The
software GraphPad Prism®, version 5.01(Graph Pad Software, Inc., California) was used for the treatment and analysis of data.