selection of microalgae with the desired phenotypes for feedstock
production. Flow cytometry combined with single-cell
sorting is a high-throughput technique for screening and
isolating microalgae from heterogeneous assemblages. Based
on the developed protocols for FSC (indication of cell size) vs.
red fluorescence (indication of CAF) or red fluorescence vs.
green fluorescence, a total of 96 strains of microalgae were
isolated from the five marine sample locations (see Table 1). As
seen in Fig. 2, R1 is a group of microalgae which attained weak
red and green fluorescence while group R2 had higher red and
green fluorescence. Red and green fluorescence of group R3
were highest, whereas red fluorescence of R4 was lowest
amongst the groups, but its green fluorescence was as high as