KITS:
Kits suitable for performing 50 assays are available from Megazyme. The
kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 5.5) and sodium azide (0.02% w/v)
as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: Phytase suspension (1.2 mL).
Stable for > 2 years at 4°C.
Bottle 3: Buffer (25 mL, pH 10.4), plus MgCl2, ZnSO4 and
sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 4: Alkaline phosphatase suspension (1.2 mL).
Stable for > 2 years at 4°C.
Bottle 5: Phosphorus standard solution (24 mL, 50 µg/mL)
and sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 6: Oat flour control powder (5 g; Phosphorus content,
see bottle).
Stable for > 5 years at room temperature.
PREPARATION OF REAGENT SOLUTIONS/SUSPENSIONS:
1. Use the contents of bottle 1 as supplied.
Stable for > 2 years at 4°C.
2. Use the contents of bottle 2 as supplied. Before opening for
the first time, shake the bottles to remove any enzyme that
may have settled on the rubber stopper. Subsequently store
the bottles in an upright position. Swirl the bottle to mix
contents before use.
Stable for > 2 years at 4°C.
3. Use the contents of bottle 3 as supplied.
Stable for > 2 years at 4°C.
4. Use the contents of bottle 4 as supplied. Before opening for
the first time, shake the bottle to remove any enzyme that
may have settled on the rubber stopper. Subsequently store
the bottle in an upright position. Swirl the bottle to mix
contents before use.
Stable for > 2 years at 4°C.
5. Use the contents of bottle 5 as supplied.
Stable for > 2 years at 4°C