Subsequently, singlecell
colonies
were loop-trans- ferred in MH broth (Sigma–Aldrich, St. Louis, MO, USA) and cultivated for 18–24 h on an orbital shaker at 37 °C. Final inoc- ulum suspensions of each test organism were adjusted in normal saline (0.9%) to 0.5 McFarland units, using the Densi- Chekä densitometer (bioMe ́rieux Inc, Marcy l’Etoile, France).
Subsequently, singlecell colonies were loop-trans- ferred in MH broth (Sigma–Aldrich, St. Louis, MO, USA) and cultivated for 18–24 h on an orbital shaker at 37 °C. Final inoc- ulum suspensions of each test organism were adjusted in normal saline (0.9%) to 0.5 McFarland units, using the Densi- Chekä densitometer (bioMe ́rieux Inc, Marcy l’Etoile, France).
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