To simulate the in vivo
dilution by saliva, the bacterial suspensions (108e109 colonies
forming units, CFU/mL (OD600 ¼ 1)) were inoculated in a sterile
electrolyte solution (SES) (0.22 g/L CaCl2, 6.2 g/L NaCl, 2.2 g/L KCl,
1.2 g/L NaHCO3) in the presence of 100 mg/L of lysozyme (SigmaeAldrich)