Viral
copies in peanut genotypes were estimated by quantitative reverse-
transcriptase polymerase chain reaction (qRT-PCR). In all,
10 symptomatic ELISA-positive plants from each peanut genotype
were evaluated by qRT-PCR using a nucleocapsid (N) genespecific
primer pair that amplified a 139-bp fragment; that is,
TSWV-NPF (5′ GCTTCCCACCCTTTGATTC 3′) and TSWVNPR
(5′ ATAGCCAAGACAACACTGATC 3′) (54).