Once the Mylar film was charged, cascade
developer was poured over it, and the Mylar film was fixed with a
more rigid transparent fixing film so that the thin Mylar would not
fold over onto itself upon removal (Fig. 1d). Sample areas of
interest were marked for ease of collection, the vacuum was turned
off, and the sample was removed (Fig. 1e). To maximize the DNA
recovered from the Mylar film, wet/dry swabbing with a
HydraFlock1 sterile flocked swab (Puritan, Guilford, ME) was
used to sample the side of the Mylar that came into contact with
the biological material (Fig. 1f).