In this study effects of autoclaving temperature (140–145 ◦C) and storing time (24, 48 and 72 h) on resistant
starch (RS) formation from high amylose corn starch were investigated and functional and pasting
properties of RS preparations were determined. High autoclaving temperature (145 ◦C) and long storing
time (72 h) showed beneficial impacts on RS formation. Significant decreases were observed in all RVA
viscosities of RS preparations as the autoclaving temperature increased. There was significant effect of
storage time on all RVA parameters of RS preparations within each autoclaving temperature. The water
binding values of RS preparations autoclaved at 145 ◦C were higher than those of the samples autoclaved
at 140 ◦C. RS preparations had approximately 2-fold higher emulsion capacity values than the
native starch. Thermal enthalpy (H) values of RS preparations were lower than those of native starch.
Autoclaving temperature and storing time had no effects on TO and TP.