2.3. Growth and algal consumption
SL and TWBW of each animal were measured every four weeks.
Three experimental units that contained algae but no abalone were used as controls for changes in algal weight. Feed was changed once a week during the growth trial and supplied in excess to guarantee ad libitum feeding along the whole experiment. To determine feed intake, freshly collected algae to be fed to the abalone were blotted dry and accurately weighed as well as the remaining algae at the end of the week. The weight of unconsumed food was deducted from the total weekly ration. Besides, weight of uneaten algae was corrected by calculating the natural change in weight of the algae in the control units during the same feeding period.
The average daily intakes by individual abalones during the entire feeding trial were calculated by dividing the algal biomass eaten each week by the feeding days and the number of abalones in each experimental unit.
The following nutritional indices were calculated for all treatments at the end of the trial:
Shell growth rate = (L2 − L1) / days of culture × 1000
Specific growth rate, SGR = (Ln W2 − Ln W1) / t × 100
Weight gain (%) = ((W2 − W1) / W1) × 100
Food conversion ratio = total feed intake (g wet) / total weight gain (g wet)
Protein efficiency ratio = (increase in body wet weight (g)) / (protein intake (g))
where L1 is the initial mean length of animals, L2 is the final mean length of animals, Ln W2 is the natural logarithm of weight at time t (days of culture), and Ln W1 is the natural logarithm of the initial weight.
2.3. Growth and algal consumption
SL and TWBW of each animal were measured every four weeks.
Three experimental units that contained algae but no abalone were used as controls for changes in algal weight. Feed was changed once a week during the growth trial and supplied in excess to guarantee ad libitum feeding along the whole experiment. To determine feed intake, freshly collected algae to be fed to the abalone were blotted dry and accurately weighed as well as the remaining algae at the end of the week. The weight of unconsumed food was deducted from the total weekly ration. Besides, weight of uneaten algae was corrected by calculating the natural change in weight of the algae in the control units during the same feeding period.
The average daily intakes by individual abalones during the entire feeding trial were calculated by dividing the algal biomass eaten each week by the feeding days and the number of abalones in each experimental unit.
The following nutritional indices were calculated for all treatments at the end of the trial:
Shell growth rate = (L2 − L1) / days of culture × 1000
Specific growth rate, SGR = (Ln W2 − Ln W1) / t × 100
Weight gain (%) = ((W2 − W1) / W1) × 100
Food conversion ratio = total feed intake (g wet) / total weight gain (g wet)
Protein efficiency ratio = (increase in body wet weight (g)) / (protein intake (g))
where L1 is the initial mean length of animals, L2 is the final mean length of animals, Ln W2 is the natural logarithm of weight at time t (days of culture), and Ln W1 is the natural logarithm of the initial weight.
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