The pH of slurry (3.5-5.5), hydrolysis temperature (30 C๐ - 60 C๐), incubationtime (8-40 h), Accellerase® 1500 (30-120 FPU/g dry weight of C. forskohlii roots) and substrate concentration (5-25% w/v) were optimized for maximum release of reducing sugars.