a b s t r a c t
Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole
plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal
worms of sheep was done that may justify their traditional use in veterinary clinical
medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME)
of both the plants was determined using mature female Haemonchus (H.) contortus and
their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo
anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0–8.0 g kg−1)
was determined in sheep naturally infected with mixed species of nematodes using fecal
egg count reduction test (FECRT) and larval counts. The study design also included untreated
as well as treated controls. Fecal egg count reduction and larval counts from coprocultures
were performed pre- and post-treatments to assess the anthelmintic activity of the plants.
CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity
and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA
and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects
on live worms as well as egg hatching. M. paradisiaca (LC50 = 2.13gmL−1) was found to
be more potent than T. portulacastrum (LC50 = 2.41gmL−1) in EHT. However, in vivo, maximum
reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with
CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg−1 on 15th day post-treatment,
respectively as compared to that of Levamisole (7.5mgkg−1) that caused 97.0% reduction
in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus
spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent,
found susceptible (P < 0.01) to the different doses of CP and CAME of both plants. The data
showed that both T. portulacastrum and M. paradisiaca possess strong anthelmintic activity
in vitro and in vivo, thus, justifying their use in the traditional medicine system of Pakistan.
© 2011 Elsevier B.V. All rights reserved
a b s t r a c t
Evaluation of anthelmintic effects of Trianthema (T.) portulacastrum L. (Aizoaceae) whole
plant and Musa (M.) paradisiaca L. (Musaceae) leaves against prevalent gastrointestinal
worms of sheep was done that may justify their traditional use in veterinary clinical
medicine. In vitro anthelmintic activity of the crude aqueous methanolic extract (CAME)
of both the plants was determined using mature female Haemonchus (H.) contortus and
their eggs in adult motility assay (AMA) and egg hatch test (EHT), respectively. In vivo
anthelmintic activity of crude powder (CP) and CAME in increasing doses (1.0–8.0 g kg−1)
was determined in sheep naturally infected with mixed species of nematodes using fecal
egg count reduction test (FECRT) and larval counts. The study design also included untreated
as well as treated controls. Fecal egg count reduction and larval counts from coprocultures
were performed pre- and post-treatments to assess the anthelmintic activity of the plants.
CAME of T. portulacastrum and M. paradisiaca showed a strong in vitro anthelmintic activity
and pronounced inhibitory effects on H. contortus egg hatching as observed through AMA
and EHT, respectively. Both plants exhibited dose and time dependent anthelmintic effects
on live worms as well as egg hatching. M. paradisiaca (LC50 = 2.13gmL−1) was found to
be more potent than T. portulacastrum (LC50 = 2.41gmL−1) in EHT. However, in vivo, maximum
reduction in eggs per gram (EPG) of faeces was recorded as 85.6% and 80.7% with
CAME of T. portulacastrum and M. paradisiaca at 8.0 g kg−1 on 15th day post-treatment,
respectively as compared to that of Levamisole (7.5mgkg−1) that caused 97.0% reduction
in EPG. All the species of gastrointestinal nematodes (GINs), i.e. Haemonchus contortus, Trichostronglyus
spp., Oesophagostomum columbianum and Trichuris ovis which were prevalent,
found susceptible (P < 0.01) to the different doses of CP and CAME of both plants. The data
showed that both T. portulacastrum and M. paradisiaca possess strong anthelmintic activity
in vitro and in vivo, thus, justifying their use in the traditional medicine system of Pakistan.
© 2011 Elsevier B.V. All rights reserved
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