A simple, sensitive, and accurate Ultraviolet Spectrophotometric method has been developed and validated for the determination of Benzene in Food Grade Hexane. Benzene in spectroscopic grade Hexane shows vibrational fine structure having four well resolved peaks. In the wavelength range 240–270 nm, peak at 255 nm is considered for the method development. Beer’s law was obeyed in the concentration range of 0.6–10.0 μL L−1, with correlation coefficient, 0.9999, detection limit 0.2 μL L−1 and quantitation limit 0.6 μL L−1 are established