This study was approved by the Board of advance study and Research (BASR), The Islamia
University of Bahawalpur and institutional ethical committee in compliance with NIH Principles of
Laboratory Animal Care 1985. The Reference No is COSM -3987/07. Statistical Analysis
The measured values obtained for different parameters (skin moisture, melanin, erythema) were
analyzed using SPSS 12.0 on the Personal computer (paired samples t-test for variation between the
two preparations; two-way ANOVA for variation between different time intervals and different
individuals).