DNA marker-assisted selection was used to
pyramid four bacterial blight resistance genes, Xa-4,
xa-5, xa-13 and Xa-21. Breeding lines with two, three
and four resistance genes were developed and tested for
resistance to the bacterial blight pathogen (Xanthomonas
oryzae pv. oryzae). The pyramid lines showed
a wider spectrum and a higher level of resistance than
lines with only a single gene. To speed up the gene
pyramiding process and to facilitate future markeraided
selection, we developed PCR markers for the
two recessive genes, xa-5 and xa-13, and used these to
survey a range of rice germplasm. The results of the
germplasm survey will be useful for the selection of
parents in breeding programs aimed at transferring
these bacterial blight resistance genes from one varietal
background to another.
DNA marker-assisted selection was used to
pyramid four bacterial blight resistance genes, Xa-4,
xa-5, xa-13 and Xa-21. Breeding lines with two, three
and four resistance genes were developed and tested for
resistance to the bacterial blight pathogen (Xanthomonas
oryzae pv. oryzae). The pyramid lines showed
a wider spectrum and a higher level of resistance than
lines with only a single gene. To speed up the gene
pyramiding process and to facilitate future markeraided
selection, we developed PCR markers for the
two recessive genes, xa-5 and xa-13, and used these to
survey a range of rice germplasm. The results of the
germplasm survey will be useful for the selection of
parents in breeding programs aimed at transferring
these bacterial blight resistance genes from one varietal
background to another.
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