2.3.3. Ursolic acid analysis
The column used was C18 column (3 μm, 4 mm × 150 mm, All C18, Agilent Technologies, France). The mobile phase was isocratic and composed of acetonitrile: 0.1% H3PO4 in water (90/10, v/v) and the flow rate was set at 0.6 mL/min. The column oven temperature was 30 °C. Run time was 15 min. 5 μL were injected. Ursolic acid was detected at a wavelength of 210 nm.