2.2. Extraction Methods Used on Guava. The leaf samples
were washed in tap water, dried, and placed into a blender
to be grounded into powder. Four solvents were arranged
in increasing polarity; n-hexane (>95%), methanol (>95%),
ethanol (>99.5%), and boiling distilled water were used for
the maceration extraction procedure. The leaf powder was
added to each of solvents to make a 20% concentration.
The mixtures were made in sterile 125mL Erlenmeyer flask
wrapped in aluminumfoil to avoid evaporation and exposure
to light for 3 days at roomtemperature.The flaskswereplaced
on a platform shaker at 70 rpm. After 3 days of soaking in
solvent, the mixtures were transferred to 50mL tubes and
centrifuged for 10min at 4,000rpm at 25∘C. The supernatant
was collected and stored at 4∘C until use.
2.2. Extraction Methods Used on Guava. The leaf sampleswere washed in tap water, dried, and placed into a blenderto be grounded into powder. Four solvents were arrangedin increasing polarity; n-hexane (>95%), methanol (>95%),ethanol (>99.5%), and boiling distilled water were used forthe maceration extraction procedure. The leaf powder wasadded to each of solvents to make a 20% concentration.The mixtures were made in sterile 125mL Erlenmeyer flaskwrapped in aluminumfoil to avoid evaporation and exposureto light for 3 days at roomtemperature.The flaskswereplacedon a platform shaker at 70 rpm. After 3 days of soaking insolvent, the mixtures were transferred to 50mL tubes andcentrifuged for 10min at 4,000rpm at 25∘C. The supernatantwas collected and stored at 4∘C until use.
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