Microbial activity in a well causes the reduction of the tetrazolium dye, which results in an increase in absorbance, as
measured by optical density. The microtiter plates were incubated at 20°C in a covered plastic container, and the
absorbance (570 nm) was read at 24- to 48-h intervals for 10 days, until mold began to grow in some of the
wells. Previous studies with Biolog plates use a shorter observation time—50 (Garland and Mills 1991) to 72 h
(Zak and others 1994). Zak and others (1994) recommended the following criteria to establish the length of
the observation period: (1) the rate of color development; (2) the number of substrates used; (3) a color change in the control well; and (4) fungi observed growing in the wells. In this study, the length of observation period was established by the first appearance of fungus growing in the wells. Our objective was to measure activity of microbial populations that might be present in small numbers, resulting in a lag time before activity is detected.
Microbial activity in a well causes the reduction of the tetrazolium dye, which results in an increase in absorbance, as
measured by optical density. The microtiter plates were incubated at 20°C in a covered plastic container, and the
absorbance (570 nm) was read at 24- to 48-h intervals for 10 days, until mold began to grow in some of the
wells. Previous studies with Biolog plates use a shorter observation time—50 (Garland and Mills 1991) to 72 h
(Zak and others 1994). Zak and others (1994) recommended the following criteria to establish the length of
the observation period: (1) the rate of color development; (2) the number of substrates used; (3) a color change in the control well; and (4) fungi observed growing in the wells. In this study, the length of observation period was established by the first appearance of fungus growing in the wells. Our objective was to measure activity of microbial populations that might be present in small numbers, resulting in a lag time before activity is detected.
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