Synergistic and cooperative interaction of extracellular lignocellulolytic
and cellulosomal enzymes are plausible mechanisms to
explain MC3F’s high cellulosic substrate degradation efficiency. The
synergistic action of lignocellulolytic enzymes has been reported
for many cellulolytic and hemicellulolytic enzyme systems, either
from aerobic or anaerobic bacteria, including the free enzymes and
cellulosomal components [43,44]. Synergism between metabolically
active cellulolytic microbes and enzymes has also been
proposed based on removal of hydrolysis inhibitory products and
surface phenomena involving adherent cellulolytic microorganisms
[45]. Further characterization of novel cellulolytic microbes