Finally, analytical figures of merit such as C/T and TTR depend on
the ability to reduce the likelihood that random events from the
food matrix may exhibit light scatter and fluorescence properties
expected for the target. The RAPID-B system for detecting E. coli
O157:H7 uses an unexpectedly large number of serial gates (6, each
2 dimensional) through which each event must pass in order to be
counted as a target cell. The rationale and efficacy of this choice are
discussed in our previous publication on this technology